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anti col1a1  (Cell Signaling Technology Inc)


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    Structured Review

    Cell Signaling Technology Inc anti col1a1
    Anti Col1a1, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/col1a1/pmc13019078-157-13-15
    Average 86 stars, based on 1 article reviews
    anti col1a1 - by Bioz Stars, 2026-09
    86/100 stars

    Images

    Related Articles

    Immunohistochemistry:

    Article Title: Vascular invasion-associated gene expression is detectable in pre-surgical biopsies of stage I lung adenocarcinoma
    Article Snippet: IHC staining for Thy1/CD90 was performed with a rabbit monoclonal antibody (D3V8A, #13801, Cell Signaling) at a 1:100 dilution after heat-induced epitope retrieval in alkaline buffer. .. IHC for COL1A1 was performed with a rabbit monoclonal antibody (E8F4L, #72026, Cell Signaling) at 1:200 dilution after heat-induced epitope retrieval in alkaline buffer. .. Primary antibody detection was performed using Discovery Hapten Detection with Discovery Anti-Rabbit HQ (#760-4815, Ventana) and visualized with the DAB chromogen (#760-124, Ventana).

    Article Title: Vascular invasion-associated gene expression is detectable in pre-surgical biopsies of stage I lung adenocarcinoma.
    Article Snippet: IHC staining for Thy1/CD90 was performed with a rabbit monoclonal antibody (D3V8A, #13801, Cell Signaling) at a 1:100 dilution after heat-induced epitope retrieval in alkaline buffer. .. IHC for COL1A1 was performed with a rabbit monoclonal antibody (E8F4L, #72026, Cell Signaling) at 1:200 dilution after heat-induced epitope retrieval in alkaline buffer. .. Primary antibody detection was performed using Discovery Hapten Detection with Discovery Anti-Rabbit HQ (#760-4815, Ventana) and visualized with the DAB chromogen (#760- 124, Ventana).

    Pyrolysis Gas Chromatography:

    Article Title: ADGRG1-targeted hypoxia preconditioned extracellular vesicles ameliorate intervertebral disc degeneration by delivering taurine to disrupt the oxidative stress feedback loop-driven ferroptosis in nucleus pulposus cells
    Article Snippet: .. Primary antibodies included FTH1(4393S, Cell Signaling Technology), COL1A1(72026T, Cell Signaling Technology), COL2A1(sc-52658, Santa Cruz Biotechnology), MMP13(ab39012, Abcam), GPX4(30388-1-AP, Proteintech), ADGRG1(sc-390192, Santa Cruz Biotechnology), TAUT (sc-393036, Santa Cruz Biotechnology), TonEBP (sc-101098, Santa Cruz Biotechnology), CDO1 (12589-1-AP, Proteintech), AMPK(10929-2-AP, Proteintech), Phospho-AMPK (Thr172)(2535T, Cell Signaling Technology), SIRT1(8469T, Cell Signaling Technology), P-SIRT1(Ser47)(2314S, Cell Signaling Technology), PGC-1α(2178S, Cell Signaling Technology), Ac-lysine(sc-81623, Santa Cruz Biotechnology), NRF2(16396-1-AP, Proteintech), TFAM(22586-1-AP, Proteintech), NCOA4(66849S, Santa Cruz Biotechnology). .. For immunoprecipitation (IP), cells were lysed in IP lysis buffer containing 150 mM NaCl, 50 mM Tris, 1% NP-40, 10 mM NaF, 20 mM β-glycerophosphate, 1 mM EDTA (pH = 8.0), and 2 mM Na 3 VO 4 (pH = 7.5), supplemented with a protease inhibitor cocktail (P8340-1 ML, Sigma-Aldrich).

    Control:

    Article Title: Ellagic Acid Attenuates MASLD by Reducing Hepatic Lipogenesis and Fibrosis in ApoE –/– Mice Fed a Cholesterol- and Cholate-Rich Paigen Diet
    Article Snippet: Metabolic dysfunction-associated steatotic liver disease (MASLD) is a chronic liver condition with a rising global incidence, closely linked to metabolic risk factors such as dyslipidemia.. Apolipoprotein E-deficient (ApoE/) mice fed a Paigen diet are an established model for MASLD and atherosclerosis research.. This study investigated the effects of ellagic acid (EA) on MASLD progression in ApoE/ mice fed a Paigen diet.

    Membrane:

    Article Title: Deciphering the synergistic mechanism of a novel flavonoid-antioxidant combination for asthma by combining systems pharmacology and experimental validation
    Article Snippet: .. The membrane was incubated with primary antibody against α-SMA (Abcam, ab7817), CDH1 (CST, 1065), COL1A1 (CST, 3395), AKT1 (CST, 2938), and GAPDH (Abcam, ab70699) over night. ..

    Article Title: Deciphering the synergistic mechanism of a novel flavonoid-antioxidant combination for asthma by combining systems pharmacology and experimental validation.
    Article Snippet: .. The membrane was incubated with primary antibody against α-SMA (Abcam, ab7817), CDH1 (CST, 1065), COL1A1 (CST, 3395), AKT1 (CST, 2938), and GAPDH (Abcam, ab70699) over night. ..

    Incubation:

    Article Title: Deciphering the synergistic mechanism of a novel flavonoid-antioxidant combination for asthma by combining systems pharmacology and experimental validation
    Article Snippet: .. The membrane was incubated with primary antibody against α-SMA (Abcam, ab7817), CDH1 (CST, 1065), COL1A1 (CST, 3395), AKT1 (CST, 2938), and GAPDH (Abcam, ab70699) over night. ..

    Article Title: Deciphering the synergistic mechanism of a novel flavonoid-antioxidant combination for asthma by combining systems pharmacology and experimental validation.
    Article Snippet: .. The membrane was incubated with primary antibody against α-SMA (Abcam, ab7817), CDH1 (CST, 1065), COL1A1 (CST, 3395), AKT1 (CST, 2938), and GAPDH (Abcam, ab70699) over night. ..

    Staining:

    Article Title: Type 2 lymphocytes restrict type 3 lymphocytes during liver fibrosis and colocalize in fibroblast niches
    Article Snippet: .. The next day, the slides were stained for 1 hour at room temperature with optimally diluted solutions of morphology markers including SYTO83 (Invitrogen, no. S11364), CD45 (Cell Signaling, no. 35154), COL1A1 (Cell Signaling, no. 72827S), and α-SMA (Abcam, no. ab184875). ..

    Bioprocessing:

    Article Title: Ginsenoside Rb1 Mitigates Myocardial Fibrosis Through Inhibiting Exosomal-Derived miRNA-21-Associated Inflammation and Cardiac Signaling.
    Article Snippet: DMEM medium, Trypsin-EDTA (0.05%), and penicillin–streptomycin, BrdU from ThermoFisher Scientific, United States. .. Primary monoclonal antibodies such as COL1A1 (#72026), COL3A1 (#66887), TGF-βI (#49728), ACTA2 (#19245), and β-actin (#4967) were purchased from cell signaling technology, Shanghai, China. ..



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    Image Search Results


    MSC-mt alleviates oxidative stress and promote tissue regeneration during wound healing (A) In vivo imaging showing the spatial–temporal persistence of fluorescently labeled MSC-mt (mtH) at the wound site at indicated time point, indicating transient but sustained early presence after topical application. (B) Measurement of ATP levels in peri-wound tissues on PWD8 showed enhanced local metabolic activity following mtH treatment. n = 5 ∼ 6 per group. (C) Quantification of malondialdehyde (MDA) levels in peri-wound tissues on PWD8 indicated reduced lipid peroxidation and oxidative stress in both MSC-mt–treated wounds. n = 5 ∼ 6 per group. (D) Laser speckle contrast imaging of blood perfusion at the wound site on PWD8 showed improved microvascular perfusion following mtH treatment. n = 5 per group. (E) Representative immunofluorescence images and quantification of CD31 expression in peri-wound tissues on PWD8, indicating enhanced angiogenesis in mtH–treated wounds. n = 6 per group. (F) Quantitative PCR analysis of angiogenesis-related gene expression in peri-wound tissues on PWD8, indicating transcriptional activation of pro-angiogenic programs following mtH treatment. n = 3 ∼ 5 per group. (G-H) Representative immunohistochemical staining and quantification of Col1a1 in wound tissues on PWD8, showing increased collagen synthesis and matrix remodeling in mtH–treated wounds. n = 6 per group. Scale bar = 100 μm. (I-J) Representative immunofluorescence staining and quantification of Vimentin and TUNEL in wound tissues on PWD8, indicating reduced fibroblast apoptosis following mtH treatment. n = 6 per group. Scale bar = 20 μm. (K-L) Representative immunofluorescence staining and quantification of Vimentin and 8-hydroxyguanosine (8-OHG) in wound tissues on PWD8, indicating attenuated oxidative DNA damage in fibroblasts following mtH treatment. n = 6 per group. Scale bar = 20 μm. Data are presented as mean ± SEM. ∗p < 0.05; ∗∗p < 0.01; ∗∗∗p < 0.001; ns, not significant.

    Journal: Materials Today Bio

    Article Title: Extracellular biogenic nanoscale mitochondria reprogram the wound microenvironment via ROS scavenging independent of cellular uptake

    doi: 10.1016/j.mtbio.2026.103023

    Figure Lengend Snippet: MSC-mt alleviates oxidative stress and promote tissue regeneration during wound healing (A) In vivo imaging showing the spatial–temporal persistence of fluorescently labeled MSC-mt (mtH) at the wound site at indicated time point, indicating transient but sustained early presence after topical application. (B) Measurement of ATP levels in peri-wound tissues on PWD8 showed enhanced local metabolic activity following mtH treatment. n = 5 ∼ 6 per group. (C) Quantification of malondialdehyde (MDA) levels in peri-wound tissues on PWD8 indicated reduced lipid peroxidation and oxidative stress in both MSC-mt–treated wounds. n = 5 ∼ 6 per group. (D) Laser speckle contrast imaging of blood perfusion at the wound site on PWD8 showed improved microvascular perfusion following mtH treatment. n = 5 per group. (E) Representative immunofluorescence images and quantification of CD31 expression in peri-wound tissues on PWD8, indicating enhanced angiogenesis in mtH–treated wounds. n = 6 per group. (F) Quantitative PCR analysis of angiogenesis-related gene expression in peri-wound tissues on PWD8, indicating transcriptional activation of pro-angiogenic programs following mtH treatment. n = 3 ∼ 5 per group. (G-H) Representative immunohistochemical staining and quantification of Col1a1 in wound tissues on PWD8, showing increased collagen synthesis and matrix remodeling in mtH–treated wounds. n = 6 per group. Scale bar = 100 μm. (I-J) Representative immunofluorescence staining and quantification of Vimentin and TUNEL in wound tissues on PWD8, indicating reduced fibroblast apoptosis following mtH treatment. n = 6 per group. Scale bar = 20 μm. (K-L) Representative immunofluorescence staining and quantification of Vimentin and 8-hydroxyguanosine (8-OHG) in wound tissues on PWD8, indicating attenuated oxidative DNA damage in fibroblasts following mtH treatment. n = 6 per group. Scale bar = 20 μm. Data are presented as mean ± SEM. ∗p < 0.05; ∗∗p < 0.01; ∗∗∗p < 0.001; ns, not significant.

    Article Snippet: For immunohistochemistry, Col1a1 expression was evaluated using a rabbit anti-Col1a1 antibody (CST, Cat# 72026, 1:200), followed by HRP-conjugated secondary antibody and DAB chromogen development.

    Techniques: In Vivo Imaging, Labeling, Activity Assay, Imaging, Immunofluorescence, Expressing, Real-time Polymerase Chain Reaction, Gene Expression, Activation Assay, Immunohistochemical staining, Staining, TUNEL Assay